FastQCFastQC Report
Sat 6 Sep 2025
SRR25630409_2_cutadapt.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR25630409_2_cutadapt.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43543075
Total Bases6.4 Gbp
Sequences flagged as poor quality0
Sequence length0-150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGATTCTTACTCCTCATCTTCTCATGACTAGTTTTCCTAACCATCATC802580.18431863160789633No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG636330.14613804835786173No Hit
CTTACACAGCACATGAACTAAAATCCACTTTGCTGTAATATTCATCGGAG538340.1236338958605932No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)